RNA Seq and Transcriptomics
Centrillion offers RNA Seq and transcriptomic analysis for both eukaryotic and prokaryotic samples. We help you determine the required coverage, identify the number of samples needed, advise on or perform RNA isolation, prepare libraries, and perform sequencing and bioinformatics analysis. Laboratory analytics are carried out throughout isolation and library preparation biochemistry steps to ensure that high quality data is produced.
Getting exceptional results for transcriptome studies is dependent on good quality total RNA, and efficient depletion of rRNAs. Centrillion’s scientists are experts at handling RNA and will work closely with you to quality check your total RNA providing quality metrics for review. Accurate quantification of RNAs is essential to optimizing cluster density and maximizing total sequence yield.
RNA-Seq and transcriptome projects begin with total RNA extraction usually followed by library construction. Depending on the type and quality of your starting material, the preparation steps may vary. Generally total RNA is isolated and then rRNA depletion is carried out. cDNA is made from mRNA, adaptors annealed, libraries pooled and sequenced. High quality sequence data is aligned to reference data and comparisons between samples e.g. controls vs. cases, are carried out. See work flow below.