Reduced Representation Bisulphite Sequencing (RRBS)
Reduced Representation Bisulphite Sequencing (RRBS) is a practical and cost-effective method designed to efficiently yield genome-wide quantitative DNA methylation information at single base resolution. Enriching for areas of the genome with high CpG content reduces the number of sequencing reads required which significantly reduces the cost of methylome analysis compared to whole genome bisulphite sequencing approaches.
Low base diversity resulting from restriction digestion and enrichment is a major challenge in obtaining excellent targeted bisulfite sequencing results. The Ovation(R) RRBS Methyl-Seq System addresses this problem by incorporating a variable number of adaptor-derived random bases at the beginning of the read to introduce sufficient diversity to ensure efficient clustering and run initiation aswell as supporting de-phasing the sequence data.
Centrillion will help you determine the required coverage, identify the number of samples needed, and advise on the number of samples that can be multiplexed per lane. Laboratory analytics are carried out throughout the process to ensure that high quality data is produced.